Abstract: The contents of water- soluble and fat- soluble vitamins in aerial part of Panax notoginseng were determined by high performance liquid chromatography. Five kinds of vitamins were respectively determined in the stems and leaves and in the flowers of Panax notoginseng,but there were some differences in the kinds of vitamins between the two parts. The correlation coefficients of vitamins were more than 0.9990. The content of Folic acid in the water-soluble vitamins which were determined was highest in the two parts,and the average content was 0.1291% in the stems and leaves and 0.1910% ~0.3163% in the flowers. VK1 was the only fat-soluble vitamin which was determined in the two parts,and the average content was 0.0033% in the stems and leaves and 0.0009%~0.0012% in the flowers. The method of this research was simple and accurate. The result could be used to lay the foundation for the research and development of the aerial part of Panax notoginseng as new resource food.