Abstract: The Liupao tea extract were cultured with fecal in vitro to investigate the effects of Liupao tea extract on intestinal flora in hyperlipidemia mice.The results showed that the number of total anaerobic incresed significantly compared with normal group with the extension of the fermentation time.While the number of total aerobic changed significantly after fermentation 36 h and significantly decreased 8.4% compared with fermentation 0 h.Among them, the number of Bifidobacterium, Lactobacillus, Bacteroides and Enterococci showed no significant diffirence compared with normal group after fermentation 36 h, and tended to the normal group level.While the number of E.coli had shown a decreasing trend, but still higher than the normal group level.The study indicated that the Liupao tea extract could promote anaerobic and inhibit aerobic bacteria in fat mice intestinal flora, regulate disorder of intestinal flora in high- fat diet- induced mice.