Abstract: In this paper, the absorption characteristics of Lactobacillus acidophilus, Streptococcus thermophilus and Bifidobacterium to Cr6 +、Cd2 +、Pb2 +under the simulated intestine environment were studied. The results indicated the Lactobacillus acidophilus had strong adsorption and enrichment to Cr6 +, and the adsorption ratio was 70% and the adsorption amount was 0.0021 mg / g.Streptococcus thermophilus also had strong adsorption and enrichment to Cr6 +and Cd2 +, and the adsorption ratio was 50% and 62%, and the adsorption amount was 0.0015 mg /g and0.1057 mg / g, respectively.Bifidobacterium also had strong adsorption and enrichment to Cr6 +and Cd2 +, and the adsorption ratio was 66.6% and 69.9%, and the adsorption amount was 0.0019 mg / g and 0.1298 mg / g, respectively.There was no obvious adsorption of Pb2 +among the three kinds of intestinal probitics under simulated intestinal environment.