Abstract: Plant endophytic fungi have the characteristics of special living environment and variety. By a large number of isolation and screening to obtain valuable endophytic fungi, it can provide more sources for novel natural products.In this study, 12 kinds of plants were randomly picked.18 strains of endophytic fungi were isolated by traditional artificial separation method.These endophytic fungi were identified using 28 S r DNA sequencing and phylogenies. The results showed that 18 strains of endophytic fungi mainly belonged to Cladosporium, Paramicrothyrium, Mycosphaerella, Colletotrichum, Corynespora, Sarocladium, Guignardia, Phoma, Pseudocercospora, Collembolispora, Alternaria and Glomerella sp., indicating the rich diversity of endophytic fungi.Among these fungi, there were three rare strains and a new strain of fungi.The secondary metabolites of these fungi were worth further studying.