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中国精品科技期刊2020
李健, 王金秋, 刘宁, 黎晨晨. 紫茄皮中花青素的纯化及稳定性研究[J]. 食品工业科技, 2015, (21): 133-138. DOI: 10.13386/j.issn1002-0306.2015.21.019
引用本文: 李健, 王金秋, 刘宁, 黎晨晨. 紫茄皮中花青素的纯化及稳定性研究[J]. 食品工业科技, 2015, (21): 133-138. DOI: 10.13386/j.issn1002-0306.2015.21.019
LI Jian, WANG Jin-qiu, LIU Ning, LI Chen-chen. Study on the purification and stability of anthocyanins from purple eggplant skin[J]. Science and Technology of Food Industry, 2015, (21): 133-138. DOI: 10.13386/j.issn1002-0306.2015.21.019
Citation: LI Jian, WANG Jin-qiu, LIU Ning, LI Chen-chen. Study on the purification and stability of anthocyanins from purple eggplant skin[J]. Science and Technology of Food Industry, 2015, (21): 133-138. DOI: 10.13386/j.issn1002-0306.2015.21.019

紫茄皮中花青素的纯化及稳定性研究

Study on the purification and stability of anthocyanins from purple eggplant skin

  • 摘要: 对以紫茄皮为原料提取的花青素进行大孔树脂纯化法纯化,探讨紫茄皮花青素的稳定性。通过静/动态吸附-解吸实验筛选大孔树脂、优化纯化工艺条件,在此基础上,探讨温度、p H、氧化/还原性物质、防腐剂、金属离子、糖等条件对紫茄皮中花青素稳定性的影响。结果表明:在供试树脂中,AB-8大孔树脂为紫茄皮花青素纯化的最适吸附剂,其吸附最优条件为为吸附时间1 h,上样量260 m L,吸附流速1 m L/min;最佳解析条件为,解析时间1 h,洗脱液乙醇体积分数为40%,洗脱液体积为80 m L,吸附流速1 m L/min,纯化后的花青素纯度为95.54%;高温、p H、H2O2、抗坏血酸、苯甲酸钠、Fe3+等导致紫茄皮中花青素稳定性降低。大孔树脂纯化法适合于紫茄皮花青素的纯化。紫茄皮花青素应在4℃、p H1.0且避光条件下保存,在储藏过程中应避免接触铁容器,紫茄皮花青素对H2O2、苯甲酸钠很敏感,使用过程中应该避免二者存在。此外,紫茄皮花青素对糖稳定,生产中可依情况适量添加。 

     

    Abstract: The anthocyanins extracted from purple eggplant skin as raw material of macroporous resin purification, and the anthocyanin from purple eggplant skins stability was studied.Through the static / dynamic adsorption and desorption experiments, the optimized conditions of macroporous resin and purification process were optimized, the effects of temperature, p H, oxidation / reduction substances, preservatives, metal ions, sugars on purple eggplant skin anthocyanin stability were investigated. The experimental results showed that selecting AB- 8macroporous resin as adsorbent, the optimal adsorption conditions were the adsorption time of 1 h, 260 m L of volumn, adsorption the flow rate of 1 m L / min, the desorption rate of 40%, the elution volume of 80 m L, the adsorption flow rate of 1 m L / min, and the purity of the purified anthocyanin was 95.54%.The anthocyanin in purple eggplant skin was sensitive to heat, acid, alkali, H2O2, ascorbic acid, sodium benzoate, Fe3 +., and it was stable to sugar.The purification method of macroporous resin was suitable for purification of purple eggplant anthocyanin.Anthocyanins of purple eggplant green elements should be in the 4 ℃, p H1.0 and avoid light preservation. In the process of storage, anthocyanin of purple eggplant should avoid contacting with iron container, and eggplant purple anthocyanins are very sensitive to H2O2 and sodium benzoate, which should be avoided at the same time during the process.In addition, purple eggplant anthocyanin was stable to the sugar, so the sugar can be added apprpriately.

     

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