固定化蚯蚓纤溶酶热稳定性及热失活动力学研究

Study on thermal stability and thermal inactivation kinetics of immobilized earthworm fibrinolytic enzyme

摘要: 通过纤维蛋白平板法测定蚯蚓纤溶酶在不同温度下的酶活力,对固定化和游离蚯蚓纤溶酶的热失活动力学进行初步分析。实验表明:随温度升高固定化和游离蚯蚓纤溶酶活性减小趋势逐渐增大,酶失活速率加快。当温度超过60℃时,两者失活现象明显,但固定化蚯蚓纤溶酶的热稳定性明显优于游离酶。动力学分析表明,在30⁶0℃范围内,固定化和游离蚯蚓纤溶酶热失活的表观活化能分别为130.43 k J·mol-1和116.65 k J·mol-1。

Abstract: The thermal stability of immobilized and free enzyme were investigated by determining enzyme activity at different temperatures by fibrin plate method, and the thermal inactivation kinetic of immobilized and free earthworm fibrinolytic enzyme was analyzed. The results showed that the relative activity of immobilized and free earthworm fibrinolytic enzyme was gradually decreased and the rate of enzymes rapidly was inactivated with the increasing of temperature, both the activity of free and immobilized enzyme were decreased seriously when the temprature was up to 60 ℃, but the latter showed better thermal stability.Enzymatic kinetics analysis indicated that the apparent activation energy of immobilized and free earthworm fibrinolytic enzyme were 130.43 k J·mol- 1and116.65 k J·mol- 1respectively between 30 ℃ and 60 ℃。