Abstract: Aflatoxin B1(AFB1) does great harm to human and animal health. The test of the acute toxicity of the strain had been done. The degradation tests of the AFB1 in mould maize,feed,rhubarb,semen boitae and yam had been implemented by using the strains' culture supernatant,the fermentation broth and the extracellular enzyme extract respectively. The results showed that the microbe cells didn't cause the reaction of the acute toxic if the biomass was lower than 2.56×1010CFU/m L. The results of the degradation test of the AFB1 were as follows. In maize,the degradation rates were 74.90%,82.60% and 65.40% respectively. In feed the degradation rates were 77.60%,82.50% and 71.20% respectively. In rhubarb the degradation rates were 73%,78.10% and68.40% respectively. In semen boitae the degradation rates were 76%,79.50% and 70.50% respectively. In yam the degradation rates were 65.30%,69.10% and 61.10% respectively.