Abstract: AB-8 macroporous resin was used for the separation and purification of the X. sorbifolia husk saponin,and the effects of sample solution concentration,ethanol volume fraction,eluent flow rate and Na OH mass fraction were examined for the orthogonal design. Furthermore,the operating parameters were optimized to an overall consideration of purity and recovery,when the sample solution concentration was 30 g / L,ethanol volume fraction was 70%,eluent flow rate was 2.0 BV / h and Na OH mass fraction was 0.20%,the purity of the X. sorbifolia husk saponin was 67.81%,and the recovery ratio was 64.04%. Besides,the antioxidant activity of the X. sorbifolia husk saponin was analyzed,and it was indicated that the purified X. sorbifolia husk saponin could effectively scavenge DPPH free radical.