三聚氰胺偶联抗原制备及其免疫原性分析

Preparation and immunogenicity analysis of melamine conjugate antigen

摘要: 探讨以2-氯-4,6-二胺-1,3,5-三嗪（CAAT）作为起始物,与6-氨基己酸（ACS）连接转化得到半抗原MelACS,再将其与牛血清白蛋白（BSA）偶联形成具有连接手臂的抗原BSA-ACS-Mel,用于单克隆抗体（m Ab）制备。经过液相色谱、Western blot及ELISA等抗原特性分析,证明半抗原Mel-ACS的转化效率达到了85.9%,且针对Mel特异性单抗获得率达到了42.9%,抗原BSA-ACS-Mel免疫原性较好,完全可以适用于三聚氰胺分子的特异性检测需要。这对其余小分子抗原的合成也起到一定的指导作用。

Abstract: The starting material 2- chloro- 4, 6- diamine- 1, 3, 5- triazine ( CAAT) reacted with 6- aminocaproic acid ( ACS) to create a hapten Mel- ACS, then this hapten was conjugated with bovine serum albumin ( BSA) to form BSA- ACS- Mel, which was supposed to have a connection arm and would be used as an antigen for the preparation of monoclonal antibody ( m Ab) . Antigenic characteristics analysis by HPLC, Western blot and ELISA proved that the yield of hapten Mel- ACS reached 85.9% and specific monoclonal antibodies against Mel reached42.9%.The antigen BSA- ACS- Mel had good immunogenicity and can be applied to detect melamine molecule specifically.This method played a guiding role in the synthesis of other small molecule antigens.