Abstract: The optimal extraction condition of pectinesterase from apple was studied. Through single factor and orthogonal experiment, Na Cl solution concentration, p H, extraction temperature and time were investigated, and the enzyme properties from apple were also studied in this article. The results showed that:under the conditions of Na Cl solution concentration 2.5mol/L, p H8.5, extraction temperature 30℃, extraction time 27 h, pectinesterase activity could reach 0.98μmol/min·m L. The optimal temperature and p H of pectinesterase was 40℃ and 11, respectively. Its temperature stability was poor. Metal ions such as Na+, Ca2 +, K+, Fe2 +could stimulate pectinesterase activity, especially Ca2+was more remarkable stimulation than others. Ba2+, Mn2+and EDTA were inhibitors of pectinesterase activity.