Abstract: Objective:To study the relief of physical fatigue effectiveness of Oviductus Ranae fermented milk.Methods:Mice were divided into high, medium and low does groups and one control group. Loading swimming test, levels of blood urea nitrogen, liver glycogen and blood lactate were detected to test the pharmacological activity of Oviductus Ranae fermented milk. Results:The swimming test showed positive result, blood lactate, blood urea nitrogen two biochemical indicators were positive, negative test results on glycogen. Conclusion:Oviductus Ranae fermented milk had effect on physical fatigue relief, it was a good health care product for the modern family.