Abstract: The content of As、Pb、Cd、Cr、Cu in four kinds of edible fungi (Auricularia auricular, Lentinus edodes, Flammulina velutipes and Grifola frondosa) was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) . Chemical speciation of heavy metals and As in samples was carried out by Tessier sequential extraction procedure. It was shown that the metal content was within most of the national standards.As in four kinds of edible fungi was mainly in forms of exchangeable, the chemical speciaion of Pb in four kinds of edible fungi was quite different, Cd in other three kinds of edible fungi was mainly in forms of water soluble and exchangeable except Flammulina velutipes, the chemical speciaion of Cr in four kinds of edible fungi was quite different, Cu in other three kinds of edible fungi was mainly in forms of water soluble except Auricularia auricular. This article showed that the Tessier sequential extraction procedure could be used for the chemical speciation of heavy metals and As in edible fungi satisfactory, and reflected the distribution information of chemical speciation.