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中国精品科技期刊2020
黄劲松, 郭军, 蒋林惠, 宦晨, 郁志芳. 6-苄氨基腺嘌呤对蕨菜木质化的影响[J]. 食品工业科技, 2015, (09): 327-330. DOI: 10.13386/j.issn1002-0306.2015.09.062
引用本文: 黄劲松, 郭军, 蒋林惠, 宦晨, 郁志芳. 6-苄氨基腺嘌呤对蕨菜木质化的影响[J]. 食品工业科技, 2015, (09): 327-330. DOI: 10.13386/j.issn1002-0306.2015.09.062
HUANG Jin-song, GUO Jun, JIANG Lin-hui, HUAN Chen, YU Zhi-fang. Effect of 6- benzyl aminopurine on postharvest lignification on Pteridium aquilinum[J]. Science and Technology of Food Industry, 2015, (09): 327-330. DOI: 10.13386/j.issn1002-0306.2015.09.062
Citation: HUANG Jin-song, GUO Jun, JIANG Lin-hui, HUAN Chen, YU Zhi-fang. Effect of 6- benzyl aminopurine on postharvest lignification on Pteridium aquilinum[J]. Science and Technology of Food Industry, 2015, (09): 327-330. DOI: 10.13386/j.issn1002-0306.2015.09.062

6-苄氨基腺嘌呤对蕨菜木质化的影响

Effect of 6- benzyl aminopurine on postharvest lignification on Pteridium aquilinum

  • 摘要: 为了有效地抑制采后蕨菜木质化,延长蕨菜的货架期。分别比较25mg/L 6-苄氨基腺嘌呤(6-benzyl aminopurine,6-BA)及50mg/L 6-BA浸泡处理蕨菜15min后,在1315℃、相对湿度80%85%条件下贮藏9d,用清水浸泡作CK,测定苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、4-香豆酸辅酶A连接酶(4-CL)、肉桂醇脱氢酶(CAD)、过氧化氢酶(CAT)等酶及丙二醛(MDA)含量、木质素含量、蛋白质含量。结果表明:6-BA处理能明显降低与木质素合成相关酶PAL、POD、4-CL、CAD等活力(p<0.05);各处理中木质素含量在贮藏期间均呈上升趋势,蛋白质含量呈下降趋势;在贮藏期间,6-BA处理组中木质素及MDA的含量低于CK组;6-BA处理及CK组蛋白组含量均呈下降趋势,但6-BA处理明显抑制蛋白质含量下降和明显提高CAT酶活力。综上50mg/L 6-BA能较好地抑制采后蕨菜的木质化。 

     

    Abstract: In order to effectively restrain post- harvest lignification and extend the shelf life of Pteridium aquilinum,Post- harvest Pteridium aquilinum was soaked in the solution of 25 mg / L and 50 mg / L 6- BA solution for 15 min; then stored under 13~ 15℃,RH 80% ~ 85% for 9d and the control( CK) was soaked in water and then as same as6- benzyl aminopurine( 6- BA) treatment. The enzyme activity of phenylalnine ammonialyase( PAL),peroxidase( POD),4- coumarate Co A ligase( 4- CL),Cinnamyl alcohol dehydrogenase( CAD),Catalase( CAT) and malondialdehyde( MDA) content,lignin content and protein content were determined.Results showed that enzyme activity of PAL,POD,4- CL,CAD treated by 6- BA was significantly lower than CK. Lignin content was ascending and protein content was descending during its storage period. Lignin and MDA content of 6- BA treatment was lower than CK.Protein content and enzyme activity of CAT was significantly higher than the control. In conclusion,the lignification process could be inhibited by 6- BA during storage periods.

     

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