银杏愈伤组织黄酮提取条件的正交实验优化

Optimization of extracting conditions of flavonoids from Gingko Callus by orthogonal experiment

摘要: 以银杏愈伤组织为材料,对银杏黄酮的提取条件进行优化。通过单因素实验研究乙醇浓度、液料比、微波功率、微波时间、水浴温度、水浴时间等对黄酮得率的影响,并进行了正交实验优化。正交实验的结果表明:以40%乙醇为提取溶剂,液料比25∶1,在中火档(528W)微波60s,不进行水浴处理,黄酮得率达到20.92mg/g。

Abstract: Using Ginkgo callus as material,the optimization of the extraction conditions of Ginkgo flavonoids was done.Through single- factor tests,the influences of several factors on extraction rate of Ginkgo flavonoids were investigated,such as ethanol concentration,liquid- powder ratio,microwave power,microwave processing time,water bath temperature,water bath processing time,etc. Then,orthogonal test was carried out. The results of orthogonal experiment indicated that: using 40% ethanol as the extraction solvent,liquid- powder ratio 25 ∶ 1,microwave processing 60 s under middle power level( 528W),no water bath treatment,the extraction rate of flavonoids reached 20.92 mg / g.