Abstract: Objective:The aim of this research was to study polyamide purification technology of flavonoids from star aniseed residue. Methods: With the absorption and desorption capacity as indexes,the optimal mesh of polyamide purification for flavonoids was determined from star aniseed residue through static absorption and desorption experiment,the adsorption conditions were optimized from single factor and orthogonal design experiment,the desorption conditions were optimized from dynamic desorption experiments. Results:The optimal purification technology conditions :sample concentration was 0.05g/m L(Crude drug concentration),p H5.0,height-diameter ratio of column 12∶1,flow rate 1~2BV/h,saturated adsorption volume 7.5~8BV,after being saturated adsorption,the polyamide resin column was washed with distilled water until Molish were negative,with 4BV 90% ethanol elution volume,at desorption rate of 70.77%,collect flow of fluid,recycling ethanol at60℃,the purity of flavonoids from dry matter medium was 87.5%. Conclusion:Purified effect of flavonoids from star aniseed residue by polyamide resin was effectively and the finally purity of the flavonoids obtained was high,it was suitable for industrial production.