响应面法优化荸荠淀粉酶解工艺及动力学研究

Enzymolysis technologies and kinetics of Chinese water chestnut(Eleocharis tuberosa) with response surface method

摘要: 以葡萄糖当量值（DE）为考察指标,利用单因素和响应面法对α-淀粉酶酶解荸荠淀粉工艺进行优化,并计算相应酶解动力学参数。结果表明:α-淀粉酶酶解荸荠淀粉优化工艺为:时间88min、温度53℃、E/S=0.12U/mg和p H7.7。在此条件下,验证实验DE为（53.393%±0.899%）。在p H7.0,60℃条件下,Km=47.298mg/m L,Vmax=0.335mg/（m L·min）,Ea=11.995k J/mol,△H=71.882k J/mol。

Abstract: With dextrose equivalent value(DE) as an index,response surface method was used to investigate the optimum enzymolysis technologies of Chinese water chestnut(Eleocharis tuberosa) starch by alpha-amylase based on single experiment,and its corresponding kinetics parameters were also established. The results indicated that the optimal technologies were time 88 min,temperature 53℃,E/S=0.12U/mg and p H7.7. The verification results of DE reached(53.393%±0.899%) under above conditions. Under p H7.0 and temperature 60℃,Kmand Vmaxwere47.298mg/m L and 0.335mg/(m L·min),respectively,Eaand △H were 11.995 k J/mol and 71.882 k J/mol,respectively.