Abstract: MSG-like components including free amino acids and 5'-nucleotides in three commercial Lentinula edodes(flower mushroom,winter mushroom and money mushroom) collected from five different areas were analyzed by amino acids analyzer and high performance liquid chromatography(HPLC),and evaluated by Taste Activity Value(TAV) and Equivalent Umami Concentration(EUC). The results showed that the content and effect in the same kinds of dried mushrooms from different areas both showed significant differences,and also in the same origin dried mushrooms of different species;total content of amino acids showed that flower mushroom produced in Fujian Ningde contained higher(7.76±0.05)mg/g,winter mushrooms produced in Henan Xixia higher(6.22±0.04)mg/g,money mushrooms produced in Zhejiang Qingyuan higher(7.38±0.03)mg/g,in which the flavor of amino acids(glutamic acid and aspartic acid) levels were highest,accounting for 11.8%~39.9% of total amino acids,and the highest concentrations of Zhejiang Qingyuan mushrooms money was(2.81±0.02)mg/g;content of nucleotide showed cytidylate had the highest levels,inosine monophosphate acid and xanthosine monophosphate acid were not detected,in which umami 5'-nucleotide levels(AMP and GMP) accounted for13.9%~48.9% of the total,and flavor nucleotide content of money mushroom from Zhejiang Qingyuan amounted to(6.45±0.21)mg/g;Taste activity value(TAV) measured values were displayed in the flavor of mushrooms flavor ingredients guanylate most significant contribution,followed by glutamate and adenosine,sweet flavor ingredientalanine most significant contribution,not taste bitter components contribute significantly(TAV <1);Equivalent umami concentration(EUC) showed Zhejiang Qingyuan money mushroomEUC=(2.033±0.07)g MSG/100 g dry matter was the most obvious effect,followed Xixia mushroomEUC=(1.618±0.13)g MSG/100 g dry matter. The results provided a theoretical basis for further practical screening enriched mushroom flavor ingredients.