Abstract: After being separated from the Tilapia`s steaks by suspension,the fishbone were dried and grinded to fishbone power. The acid value and peroxides value of fishbone powder were analyzed,and then the fishbone powder was degreased by Soxhlet extraction.The content of minerals in fishbone powder and leachate of different meshes fishbone powder in simulated gastric fluid,such as calcium,iron,zinc,lead,cadmium,arsenic and selenium,were determined by Atomic emission spectrometry( AES). The different meshes fishbone powder was used to make cookies and sensory evaluation test was conducted. Results showed that,the acid value and peroxides value of fishbone powder were increased in storage,and the degreasing should be added to fishbone powder production.There were abundant minerals in fishbone powder,especially calcium.The smaller the fishbone powder was,the better digestion effect of mineral elements and cookies` sensory were. The fishbone powder with100 meshes was better for food production.