Abstract: The response values of the odor of durian pulp were detected by PEN3 portable E-nose.The method of sealing and punching was applied to make the signal curve reach steady much faster.The total contribution of the first and the second principal component of the durian pulp was 99.49% by PCA,and the odors of the three samples were obviously different.It was feasible to identify the differences between the odors with different levels of durian pulp by E-nose. The result of loadings was that sulfide was the main component. According to the data detected,a model of measuring the relative content of the odor was established by the method of PLS and a linear fitting curve of which the correlation coefficient was 0.997 was drawn up by statistical software. This showed that there was a good linear relationship between the relative content of the odor and the output signal of E-nose.The average relative errors of the samples with 0 hole,8 holes and 16 holes were 6.3%,6.5% and 6.7%.Therefore,in this paper,the model can accurately determine the relative content of the odor of durian pulp so as to provide a practical method to evaluate durian odor.