采后BTH处理对苹果果实苯丙烷代谢和病程相关蛋白积累的增强作用
Activation of postharvest BTH treatment on phenylpropanoid pathway and pathogenesis- related proteins accumulation in apple fruit
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摘要: 本文探讨采后苯丙噻重氮（BTH）处理对苹果果实苯丙烷代谢和病程相关蛋白产生的增强作用。以"国光"苹果为试材,采后用100mg/L BTH浸泡处理10min,测定处理对果实苯丙烷代谢相关酶活性、产物积累和病程相关蛋白产生的影响。结果表明,BTH处理显著提高了苹果果实体内苯丙氨酸解氨酶（PAL）、4-香豆素辅酶A连接酶（4CL）的活性,增加了总酚、类黄酮及木质素含量,同时提高了β-1,3葡聚糖酶（GLU）、几丁质酶（CHT）和过氧化物酶（POD）的活性。由此表明,采后BTH处理可通过诱导果实苯丙烷代谢和促进病程相关蛋白的产生来增强果实对采后病害的抗性。Abstract: The effect of benzothiadiazole( BTH) dipping on phenylpropanoid pathway and accumulation of pathogenesis- related proteins in apple fruit was investigated in this paper.Apple fruit( cv.Guoguang) were dipped in 100 mg / L BTH for 10 min to study the effect of BTH on key enzymes activities,metabolites accumulation in phenylpropanoid pathway and production of pathogenesis- related proteins( PRs). The results indicated that BTH treatment significantly increased the activities of phenylalanine ammonia- lyase( PAL) and 4- coumaric acid-coenzyme A ligase( 4CL),and the content of total phenolic compounds,flavonoids and lignin.The activities of β-1,3- glucanase( GLU),chitinase( CHT) and peroxidase( POD) were also induced by BTH treatment. These results suggest that postharvest BTH treatment induced disease resistance in apple fruit by activating phenylpropanoid pathway and promoted the accumulation of PRs.
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