Abstract: Oils were extracted from white semen sesami nigrum,white peanut,black peanut,sunflower seed,soybean and cotton seed by Soxhlet extraction with mineral ether.Oil samples were dissolved with normal hexane and being a constant volume with methyl alcohol,the content of normal hexane was 20%( v / v) in the solution.The contents of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E in the extracted oils were determined on a ODS2 column,using methyl alcohol as mobile phase. Experimental results showed that linear relations of the concentrations of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E to peak areas were obtained within0~200μg / m L at 204 nm. The correlation coefficients were more than 0.9978. The average recoveries of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E were 97.83%,98.58%,98.75% and 99.93%,respectively.