Abstract: Objective To study the effects of Seabuckthorn on activities of enzymes and content of trace elements in the testis of mice after acute alcohol administration. Methods Male ICR mice were divided randomly into five groups,including control group,alcohol model group and 0.6,3,6g / kg Seabuckthorn groups.Continuous administration for 14 days,since fifteenth,the daily after administration of 1h,alcohol model group and Seabuckthornin different dosage groups with 40% alcohol by Intragastric 15 m L / kg,end to twentieth days.The testis of mice were measured,lactate LDH,ACP,AKP activity and trace elements copper,zinc,iron content.Results The activity of LDH compared with the alcohol model group testicular tissue,seabuckthorn low dose group,increased significantly( p < 0.05); the activity of ACP in the testis,Hippophae rhamnoides,low and high dose group was obviously lower than the alcohol model group( p < 0.05); copper content of testicular tissue of seabuckthorn in the high dose group was significantly higher than the alcohol model group( p < 0.05).Conclusion The activity of LDH in testis of acute alcohol poisoning of mice was increased,the activity of ACP was reduced,the content of copper was increased by adding seabuckthorn juice.