Abstract: In order to obtain an industrial strain with higher thermostable protease production,the original strain of Bacillus licheniformis TP1 was mutated by atmospheric and room temperature plasmas. A good genetic stability strain TP1- 5 was screened from the mutants,which produced 33200 U / m L from initial 21300 U / m L of the thermostable protease.The protease was purified by ethanol precipitation and anion exchange chromatography to electrophoretic homogeneity. The purified protease had a good p H stability and thermal stability,with optimal temperature and p H was 60℃ and 10.0. The protease also exhibited high stability towards surfactants such as SDS,Tween-80,Triton X-100 et al.