Abstract: Neutral and alkali protease were used for hydrolyzing chicken protein to test their proliferative effects on Lactic acid bacteria ( LAB) . Hydrolysates from neutral and alkali protease hydrolyzation with different hydrolytic time (2, 4 and 6h) by 1%、2%、3% dosage were added to MRS broth medium with four kinds of LAB (S. thermophilus, L. bulgaricus, L. acidophilus, L. Casei) . The different proliferative effects of hydrolysates on LAB were determined by measuring absorbancy OD622 after culturing for 72 hours at 37℃. The results showed that neutral and alkali protein hydrolysates had great proliferative effects on LAB. Under the same hydrolysis time, the Neutral and alkali hydrolysates had maximum proliferative effects with the inoculation amount of 3%.Under the same inoculation amount, the hydrolysates had maximum proliferative effects with hydrolyzation for6 h by protease.