Abstract: Aimed at using bacteriophage as natural anti- bacteria, this study isolated a lytic Vibrio parahaemolyticus phage through sewage from aquatic market by double- layer method and it was designated as SHOU24, and p H and thermal stability were tested. Phage SHOU24 was specific lytic to Vibrio parahaemolyticus with tdh gene. The phage belonged to Siphoviridae by transmission electron microscope ( TEM) . The most suitable p H for phage SHOU24 was 7⁸ while it could live at p H between 3¹1.The survival rate was high under 50℃ condition.In antibacteria on ready- to- eat shrimp experiment, the bacteria amount dropped by 1log CFU / g at 25℃. This study provided theory foundation for using phage SHOU24 as bacterial biocontrol agent.