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中国精品科技期刊2020
黄永光, 徐岩. 真菌Aspergillus hennebergii酸性蛋白酶小麦固态发酵性能研究[J]. 食品工业科技, 2014, (21): 135-139. DOI: 10.13386/j.issn1002-0306.2014.21.021
引用本文: 黄永光, 徐岩. 真菌Aspergillus hennebergii酸性蛋白酶小麦固态发酵性能研究[J]. 食品工业科技, 2014, (21): 135-139. DOI: 10.13386/j.issn1002-0306.2014.21.021
HUANG Yong- guang, XU Yan. Study of the performance of acid protease from Aspergillums hennebergii in solid- state fermentation of wheat[J]. Science and Technology of Food Industry, 2014, (21): 135-139. DOI: 10.13386/j.issn1002-0306.2014.21.021
Citation: HUANG Yong- guang, XU Yan. Study of the performance of acid protease from Aspergillums hennebergii in solid- state fermentation of wheat[J]. Science and Technology of Food Industry, 2014, (21): 135-139. DOI: 10.13386/j.issn1002-0306.2014.21.021

真菌Aspergillus hennebergii酸性蛋白酶小麦固态发酵性能研究

Study of the performance of acid protease from Aspergillums hennebergii in solid- state fermentation of wheat

  • 摘要: 传统固态发酵酿造是多菌系、多酶系、多物系、多变性的多维发酵系统。在整个发酵酿造的酸性环境中,酸性蛋白酶对蛋白水解、微生物生长和风味成分的形成与调控起着举足轻重的作用。以小麦为原料,考察Aspergillus hennebergii产胞外酸性蛋白酶对小麦固态发酵性能的影响。结果表明,与不添加酸性蛋白酶的对照样相比,添加酸性蛋白酶可使小麦固态发酵中蛋白水解度提高28.26%,氨基酸态氮含量提高34.21%,S.cerevisiae生物量提高37.09%,提高总酸36.17%,提高酒醅乙醇含量38.29%;300u以下小麦蛋白水解产物提高达14.1%,可促进芳香族氨基酸代谢,产生更多的风味成分。 

     

    Abstract: Traditional solid- state fermentation is multi- dimensional variability fermentation system including microbial diversity, enzyme diversity, substances diversity, metabolic diversity etc.Acid protease plays an important role for hydrolyzing protein, increasing microbial biomass and forming flavour compounds during the whole fermenting process. In this study, wheat was used as raw materials for solid- state fermentation, and the performance of extracellular acid protease from Aspergillus hennebergii was investigated.The results showed that, compared with the control samples without any addition of acid protease, the addition of acid protease could increase hydrolysis degree of wheat protein by 28.26%, increase amino acid contentration of fermented grains by34.21%, promote S. cerevisiae biomass of fermented grains by 37.09%, increase total acids content of fermented grains by 36.17%, enhance alcohol content by 38.29% in fermented grains, and increase wheat protein hydrolyzate ( less than 300u) to 14.1 % which could promote the metabolism of aromatic amino acid and produce more flavour compounds.

     

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