影响枯草芽孢杆菌B-1发酵南极磷虾壳的因素研究

Study on the influencing factors of fermentation of Antarctic krill shells with Bacillus subtilis B-1

摘要: 以高产蛋白酶的枯草芽孢杆菌B-1菌株发酵南极磷虾壳,脱除虾壳中的蛋白质。研究了外加碳源、料液比等因素对发酵过程中枯草芽孢杆菌B-1产蛋白酶活力的变化及虾壳蛋白去除率的影响,并利用正交实验对影响虾壳蛋白去除率的发酵条件进行优化,确定了最佳发酵条件为发酵时间72h,发酵温度37℃,接种量为4%,料液比1∶3,此条件下蛋白质去除率为88.9%。

Abstract: Bacillus subtilis B-1 was used as fermentation strains to hydrolyze protein from Antarctic krill shells in this study.Further to remove protein from krill shell waste, the variation rules of protease activity of Bacillus subtilis B-1and the optimized condition of deproteinization in the process of fermentation were analyzed. The result showed that the best fermentation conditions were 72 h, 37℃, 4% of inoculums quanity and 1 ∶ 3 of the ratio of material to liquid.Liquid phase fermentation of krill shell waste showed protein removal of 88.9%.