碱性蛋白酶酶解提取羊血血红素工艺条件优化

Process optimization of the alkaline enzymatic extracting heme from sheep blood

摘要: 以羊血为试材,血红素得率为指标,在加酶量、酶解pH、酶解温度和酶解时间等单因素实验基础上,采用正交实验优化工艺参数。结果表明最佳反应参数为碱性蛋白酶加酶量8000U/g血红蛋白、酶解pH8.5、酶解温度55℃、酶解时间4h,在此条件血红素得率达到85.3%,即1L羊血可提取3.93g血红素。该法所得产品的安全性高,适合工业化生产。

Abstract: Sheep blood as the research material and the yield of heme was measure indicate. Orthogonal array design generated based on single-factor experiments was employed to optimize conditions. The extraction factors such as the amount of enzyme, pH, react temperature and react time were investigated. The results showed that the optimal amount of enzyme was 8000U/g, pH8.5, react temperature was 55℃, react time was4 h, under these optimal conditions, the yield of heme was 85.3%, that was equivalent to 3.93 g heme of 1L sheep blood. This method had higher safety than others, it was suitable for industrialized production.