一株木质纤维素酶高产菌株的选育及产酶条件研究

The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production

摘要: 主要通过紫外(UV)和甲基磺酸乙酯(EMS)的交替诱变的方法提高绿色木霉的产酶能力,并成功筛选到了一株产酶活性较高的菌株,其在平板筛选培养基中的透明圈直径与菌落直径之比达到2.1,传代稳定后在产酶培养基中纤维素酶活(CMCase)和滤纸酶活(FDAase)分别达到21.05U/mL和2.4U/mL。之后经培养基的优化进一步提高其酶活,最终诱变菌株在最佳产酶培养基中CMCase达到22.5U/mL,FDAase达到2.52U/mL。

Abstract: The cellulase production strain T. viride 8140 was treated with UV and EMS. The mutant strain T.viride8140UEUE4-80 was showed to have a higher cellulase activity than others and the Hc of the mutant reached 2.1. After several passages its CMCase and FDAase were stabilized at 21.05U/mL and 2.4U/mL. The conditions of fermentation were studied and the optimal culture medium was decided. Under the optimal culture medium, the CMCase and FPAase of the mutant separately reached 22.5, 2.52U/mL.