Abstract: Tilapia skin collagen polypeptides (TSGP) was prepared and chelated by iron. The physical, chemical and functional properties of the chelate were characterized. The optimum parameters of chelating as follows:mpolypeptides∶mFeCl2·4H2O=500∶1, pH6.0, reaction time 50 min, temperature 35℃. The formation of iron-TSGP chelate was confirmed by the Ultraviolet spectrum and Infrared spectroscopy, and found that the carboxyl and amino group of TSGP bounded with iron. In addition, chelate had a strong radical scavenging activities of DPPH· and ABTS+·.