Abstract: The purpose was to establish the method of ( +) - catechin in Fragaria orientalis Lozinsk. The samples were performed on Agilent ZORBAX SB- C18 ( 4.6mm × 150 mm, 5μm) column, eluted gradiently with mixture of acetonitrile-0.1% phosphate solution at a flow rate of 1mL /min, and detected at 280 nm.With this method, ( +) -catechin in 10 batches of F. orientalis Lozinsk. was determined. Results showed that ( +) - catechin retention time was 14.543 min.The calibration curves had good linearity within the range of 0.0512 ~ 0.6400μg ( R2= 0.9999) . The average recovery of ( +) - catechin was 99.70% ( RSD 2.67%) . The method was easy and stable, and could be used for the quantitative analysis of F.orientalis Lozinsk.