UPLC-MS/MS检测原料奶中脱呋喃甲酰基头孢噻呋残留方法的建立

赵静 刘鹏 钱镭

赵静, 刘鹏, 钱镭. UPLC-MS/MS检测原料奶中脱呋喃甲酰基头孢噻呋残留方法的建立[J]. 食品工业科技, 2014, (16): 92-95. doi: 10.13386/j.issn1002-0306.2014.16.011
引用本文: 赵静, 刘鹏, 钱镭. UPLC-MS/MS检测原料奶中脱呋喃甲酰基头孢噻呋残留方法的建立[J]. 食品工业科技, 2014, (16): 92-95. doi: 10.13386/j.issn1002-0306.2014.16.011
ZHAO Jing, LIU Peng, QIAN Lei. Establishment on UPLC-MS/MS detection methods for desfuroylceftiofur in raw milk[J]. Science and Technology of Food Industry, 2014, (16): 92-95. doi: 10.13386/j.issn1002-0306.2014.16.011
Citation: ZHAO Jing, LIU Peng, QIAN Lei. Establishment on UPLC-MS/MS detection methods for desfuroylceftiofur in raw milk[J]. Science and Technology of Food Industry, 2014, (16): 92-95. doi: 10.13386/j.issn1002-0306.2014.16.011

UPLC-MS/MS检测原料奶中脱呋喃甲酰基头孢噻呋残留方法的建立

doi: 10.13386/j.issn1002-0306.2014.16.011
详细信息
    作者简介:

    赵静 (1989-) , 女, 在读硕士研究生, 研究方向:乳品质量与安全。;

  • 中图分类号: O657.63;TS252.7

Establishment on UPLC-MS/MS detection methods for desfuroylceftiofur in raw milk

  • 摘要: 目的:建立超高压液相色谱-串联质谱法(UPLC-MS/MS)测定原料奶中脱呋喃甲酰基头孢噻呋来间接衡量头孢噻呋残留的方法。方法:利用T3色谱柱,以含有0.02%的甲酸水-乙腈溶液为流动相进行梯度洗脱,采用二硫赤藓糖醇将原料奶中头孢噻呋分解为脱呋喃甲酰头孢噻呋(DFC),由碘乙酰胺衍生化为稳定的DFC乙酰胺形式(DCA),样品再经HLB固相萃取柱富集净化后,进行UPLC-MS/MS检测。结果:m/z 430.12>125.79为定量离子对,m/z 430.12>241.04为定性离子对,回收率在98.6%102.2%,精密度(RSD)为2.51%3.87%,最低检出限为0.45ng/mL。结论:该方法可用于原料奶中脱呋喃甲酰基头孢噻呋残留的测定。 
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  • 收稿日期:  2013-12-05

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