Abstract: For the rational use of Chinese soft-shelled turtle, the proximate composition of muscle ( legs and carina) was determined and protein composition was separated.Amino acid composition of leg muscle and its separated protein components was studied by amino acid analyzer. The results showed high protein content of 18.20% ( weight weight) and 20.97% ( weight weight) , low fat content with 0.96% ( weight weight) and 0.42% ( weight weight) respectively in the legs and carina.The proportion of sarcoplasmic protein, myofibrillar protein, stroma protein of the legs was 31.79%, 39.47%, 28.72%, respectively. In the carina, mainly composed of stroma protein, which accounted for 92.25%.There were some differences in the proportion of each protein amino acid.The legs and myofibrillar protein in the line with the Food and Agriculture Organization of the United Nations /World Health Organization ( FAO /WHO) standard.Compared the amino acid scores ( AAS) of each protein in the legs, we found that only in the legs, the AAS was all above 100.So, compared to its protein components, the leg muscle was a more balanced food.