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中国精品科技期刊2020
张丹, 李丹, 朱小峰, 康文艺. 木瓜抗氧化活性研究[J]. 食品工业科技, 2014, (15): 130-133. DOI: 10.13386/j.issn1002-0306.2014.15.018
引用本文: 张丹, 李丹, 朱小峰, 康文艺. 木瓜抗氧化活性研究[J]. 食品工业科技, 2014, (15): 130-133. DOI: 10.13386/j.issn1002-0306.2014.15.018
ZHANG Dan, LI Dan, ZHU Xiao-feng, KANG Wen-yi. Antioxidant activities of Chaenomeles sinensis ( Touin) Koehne[J]. Science and Technology of Food Industry, 2014, (15): 130-133. DOI: 10.13386/j.issn1002-0306.2014.15.018
Citation: ZHANG Dan, LI Dan, ZHU Xiao-feng, KANG Wen-yi. Antioxidant activities of Chaenomeles sinensis ( Touin) Koehne[J]. Science and Technology of Food Industry, 2014, (15): 130-133. DOI: 10.13386/j.issn1002-0306.2014.15.018

木瓜抗氧化活性研究

Antioxidant activities of Chaenomeles sinensis ( Touin) Koehne

  • 摘要: 采用DPPH法、ABTS法和FRAP法三种测定法对木瓜叶和木瓜果实体外抗氧化活性进行综合评价,并分析其总多酚与总黄酮含量与抗氧化活性的关系。结果表明:木瓜6个提取物中,木瓜叶乙酸乙酯部位清除ABTS自由基及还原Fe3+(IC50=6.66μg/mL,TEAC=1293.25μmol/g)最强,其中,清除ABTS自由基活性和阳性对照BHT(IC50=6.56μg/mL)相当,还原Fe3+能力低于BHT(TEAC=2503.17μmol/g);木瓜果实乙酸乙酯部位清除DPPH自由基(IC50=48.99μg/mL)能力最强,弱于阳性对照BHT(IC50=24.49μg/mL)。木瓜叶乙酸乙酯部位清除ABTS自由基及还原Fe3+的能力可能与其多酚、黄酮含量高有关,木瓜果实乙酸乙酯部位清除DPPH自由基的能力可能也与其多酚、黄酮含量高有关。 

     

    Abstract: The antioxidant activity of Chaenomeles sinensis ( Touin) Koehne leaves and fruits was evaluated by three methods: DPPH radical scavenging, ABTS radical scavenging and FRAP assay, and the relation of the antioxidant activity and total polyphenols and flavonoids was studied.In six extracts of the leaves and fruits, the ethyl acetate extract of C. sinensis leaves had the best ABTS radical scavenging ( IC50 was 6.66μg /mL) and ferric reducing antioxidant power ( TEAC was 1293.25μmol /g) , which were similar to the positive control BHT ( IC50 was 6.56μg /mL) and ferric reducing antioxidant power was lower than that of BHT ( TEAC was 2503.17μmol /g) . The ethyl acetate extract of C.sinensis fruits exhibited the highest free radical scavenging activity ( IC50 was 48.99μg /mL) , but it was still inferior to positive control BHT ( IC50 was 24.49μg /mL) . ABTS radical scavenging activity and Ferric reducing antioxidant power of ethyl acetate extract of C.sinensis leaves might be related to its high flavonoids content and polyphenols content, and DPPH radical scavenging activity of ethyl acetate extract of C. sinensis fruits might be so.

     

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