Abstract: Lactobacillus acidophilus has the capacity of cholesterol- lowering in serum and cultivate media and its mechanism mainly embodies in adsorbing and assimilating cholesterol. By using MATH method, the surface hydrophobicity, resistance to ultrasonic and composition change of cellaur fatty acid mediated by cholesterol of La-XH1 and La-XH2, were measured.The results showed that La- XH1 and La- XH2 both had stronger surface hydrophobicity to hexadecane and xylol.Scanning electron microscope showed that La- XH1 and La- XH2 could adsorb cholesterol to its surface and deposit with thallus in centrifugal condition.In addition, strain in cultivate media including cholesterol had notable anti-ultrasonic resistance compared with that grown in common MRS cultivate.GC-MC analysis found that the relative amount of celluar saturated fatty acid of Lactobacillus acidophilus La-XH1 and La-XH2 had respectively declined to 13.55% and 17.87% from 16.99% and 18.31%, and the relative amount of unsaturated fatty acid respectively rose to 53.73% and 53.91% from 33.69% and 32.22%, which changed the composition of its own cellaur fatty acid and cholesterol had been adsorbed and assimilated into its thallus.