pH响应两亲性壳聚糖衍生物纳米胶束制备、表征及对维生素D3的体外释放研究
pH-responsive amphiphilic chitosan derivative-based nanomicelles:Preparation, characterization and in vitro release of Vitamin D3
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摘要: 以羧甲基壳聚糖、N,N-二甲基十六烷基叔胺以及丙烯酸为原料合成了一种新型的pH响应性两亲性纳米材料,利用FT-IR对其结构进行了表征。该纳米材料在水溶液中能够自发形成具有壳-核结构的纳米胶束,临界胶束浓度为95mg/L,不同pH条件下粒径分布有较大差异,表面Zeta电位均为正。以维生素D3为模型分子,测得其对VD3的包封率达到57.3%,同时考察了该纳米胶束包裹VD3在不同pH条件下的释放行为,结果显示在人工模拟胃液（SGF）中累积释放量最低（42.1%）,人工模拟肠液（SIF）中最高（69.2%）。该纳米胶束的pH响应性行为表明其适宜作为VD3以及其他食品活性成分的潜在纳米生物活性载体。Abstract: A novel amphiphilic and pH-responsive nanomaterial was synthesized using carboxymethyl chitosan, N, N-dimethylhexadecylamine and acrylic acid. The structure property and critical micelle concentration of the nanomaterial was characterized. The material has the capability of forming core-shell micelles in aqueous solution with critical micelle concentration of 95mg/L. The micelles exhibited different size in diverse pH conditions, and the Zeta potential were all positive charged. Vitamin D3was used as a model molecule, and the results indicated that the solubility of VD3was improved with higher encapsulation efficiency (EE, 57.3%) . The in vitro release of VD3from the micelle in different pH solution indicated that the VD3-loaded micelle has the highest accumulative release (69.2%) in simulated intestinal fluid (SIF) , while the lowest (42.1%) in simulated gastric fluid (SGF) . This novel core-shell micelle might be a potential biological carrier for VD3or other bioactive food agents.
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