Abstract: Salmonella is a kind of common foodborne pathogens in nature which causes the greatest number of food poisoning cases in China. Female Japanese white rabbits were immunized with formalin inactivated Salmonella typhimurium as antigens to prepare polyclonal antibody. The anti-Salmonella polyclonal antibody was used as capture antibody and C1359 monoclonal antibody was used as detection antibody to develop rapid double antibody sandwich ELISA. It showed that A, B, C, D, and E typical strains of Salmonella could be detected and the limit of detection was 1 ×104CFU/mL in medium, and there was no cross reaction with miscellaneous bacteria. It could be concluded that this method was with high sensitivity and specificity.