Abstract: Predictive Microbiology is an interdisciplinary subject of mathematics, statistics and applied computer science, to make rapid risk assessment and establish predictive model for growth of pathogen and spoilage organisms under dynamics conditions. In this paper, the development and classification of predictive microbiology were summarized. The application of microbial predictive model in shelf life, risk assessment, quality and safety control of fresh-cut fruits and vegetables were introduced. Finally, current deficiencies in this field were stated.