Abstract: To investigate the relationship of the muscle fiber characteristics and glycolytic potential of Bamei lamb, longissimus dorsi (LD) , biceps femoris (BF) and supraspinatus (SS) muscles were obtained from lambs and were comparatively analyzed for improve the quality of meat. The results showed that the muscle fiber characteristics in different muscles were significantly different (p<0.05) . There was significantly difference in fiber diameter and cross-sectional area of typeⅠamong the three anatomical sites (p<0.05) . The fiber diameter and cross-sectional area of ⅡA in SS were significantly higher than that in other parts and the fiber diameter and cross-sectional area of ⅡB in LD was lower than that in other parts (p<0.05) . The glycolytic potential of LD was significantly higher than that in BF and SS. Glycolytic potential showed a significant negative correlation with typeⅠand ⅡA fiber of the percentage number and area and ⅡA fiber number percentage (p<0.05) .However, glycolytic potential showed a significant positive correlation with Ⅱ B fiber number and area percentages (p<0.05) .