Abstract: Objective:To observe the influence of wheat oligopeptides ( WOP) on cholesterol metabolism-related genes in HepG2 cells on the basis of compositions and molecular weight distribution of WOP.Method:HepG2 cells were divided into five groups in random:the control, 2, 4, 6 and 8g /L of WOP groups.After treated by WOP for 24h, RT-PCR was used to test the changes of expression of low density lipoprotein receptor ( LDLR) and 3-hydroxy-3-methylglutaryl coenzyme A reductase ( HMGCR) genes. Result:Compared with the control group, after treated by WOP, LDLR mRNA level was obviously increased in all test groups ( p < 0.05, p < 0.01) , with LDLR mRNA level in 6g / L of WOP group increased the most.HMGCR mRNA level was all decreased in all test groups.The expression of HMGCR mRNA was extremely significantly decreased in 2, 4 and 6g /L groups, with HMGCR mRNA level in 2g /L of WOP group decreased the most.Conclusion:WOP could increase LDLR mRNA level and decrease HMGCR mRNA level at all the test concentrations, therefore WOP could reduce the levels of cholesterol in HepG2 cells.The results of this paper provide experiment and theoretical basis for the development and utilization of WOP.