Abstract: Objective:Research the optimum conditions of extracting flavonoids from Gualou Xiebai Decoction. Method:Ultrasonic and microwave was used to extract the total flavonoids, and the single factor and response surface methodology was selected to optimize extract process. On the basis of single factor experiments for the ethanol concentration, such as solid-liquid ratio, time, power, etc, the optimum conditions for total flavonoids extract were obtained through Box-Behnken center united experiment designed by response surface methodology.Result:The optimum conditions were as follows, the alcohol density was 50%, the extraction time was 35min, and the solid-liquid ratio was 1∶50. Under the conditions, the extraction rate of total flavonoids could be reached to 5.168%.Microwave extraction technology condition were as follows, the solid-liquid was 1∶50, while the extract time was 1.5min and 80W, flavonoids extraction yield could be reached by 5.145%.Conclusion:Within those conditions, ultrasonic assisted extraction to extract flavonoid yield is higher.