Abstract: Objective:These optimum conditions for the microwave extraction of flavonoids from wild jackfruit root barks were determined using response surface methodology. Methods:Wild jackfruit root barks as the materials, flavonoids were extracted by microwave extraction method. On the basis of one-factor-at-a-time experiments, the process conditions of microwave extraction of flavonoids from wild jackfruit root barks were optimized by Box-Benhnken central composite test and response surface analysis method. Results:Results showed that these optimal extraction conditions were as follows:60% ethanol solution as extraction solvent, microwave extraction time of 2.2min, liquid-to-solid ratio of 52∶1mL/g. Under these conditions, the actual extraction rate of flavonoids from wild jackfruit root barks total was 9.82% in comparison with the predicted yield of 9.80%. Conclusion:The parameter of microwave extraction was optimized by response surface methodology are accurate and reliable.