Abstract: Using high performance liquid chromatography (HPLC) with Pre-column Derivation technology to study the fingerpingt chromatogram of amino acids compounds of Gynostemma pentaphylla cultivated pingli Shaanxi. Results showed that the main amino acids compounds of Gynostemma could be separated well and determined with O-phthalaldehyde as the derivatization reagent, Kromasil C18column as the chromatographic column, 0.05mol/L sodium acetate with 0.5% furanidineand and methanol as the mobile phase, by the gradient elution method. 13 samples of HPLC fingerprints showed that this method could identify a total of 22chromatographic peaks as Gynostemma pentaphylla cultivated pingli Shaanxi fingerprint peaks. The similarities were determined by the coefficients of cosine, correlation and Similarity evaluation system for chromatographic fingerprint (Version 2004 A) . Results of similarity analysis were 0.989¹.00. It could generally and exactly reflect the characteristics amino acids compounds from pingli Shaanxi. The developed method provided a new method for protecting Gynostemma pentaphylla cultivated pingli source territory.