嗜酸乳杆菌NCFM表层蛋白的提取及其与菌体酸和胆盐耐受及粘附性能的关系

胡斌 田丰伟 赵建新 张秋香 王刚 张灏 陈卫

胡斌, 田丰伟, 赵建新, 张秋香, 王刚, 张灏, 陈卫. 嗜酸乳杆菌NCFM表层蛋白的提取及其与菌体酸和胆盐耐受及粘附性能的关系[J]. 食品工业科技, 2014, (05): 118-121. doi: 10.13386/j.issn1002-0306.2014.05.080
引用本文: 胡斌, 田丰伟, 赵建新, 张秋香, 王刚, 张灏, 陈卫. 嗜酸乳杆菌NCFM表层蛋白的提取及其与菌体酸和胆盐耐受及粘附性能的关系[J]. 食品工业科技, 2014, (05): 118-121. doi: 10.13386/j.issn1002-0306.2014.05.080
HU Bin, TIAN Feng-wei, ZHAO Jian-xin, ZHANG Qiu-xiang, WANG Gang, ZHANG Hao, CHEN Wei. Extraction of Surface Layer Protein from Lactobacillus acidophilus NCFM and the relationship among SLP and strain acid, bile resistance and cell adhesion[J]. Science and Technology of Food Industry, 2014, (05): 118-121. doi: 10.13386/j.issn1002-0306.2014.05.080
Citation: HU Bin, TIAN Feng-wei, ZHAO Jian-xin, ZHANG Qiu-xiang, WANG Gang, ZHANG Hao, CHEN Wei. Extraction of Surface Layer Protein from Lactobacillus acidophilus NCFM and the relationship among SLP and strain acid, bile resistance and cell adhesion[J]. Science and Technology of Food Industry, 2014, (05): 118-121. doi: 10.13386/j.issn1002-0306.2014.05.080

嗜酸乳杆菌NCFM表层蛋白的提取及其与菌体酸和胆盐耐受及粘附性能的关系

doi: 10.13386/j.issn1002-0306.2014.05.080
基金项目: 

国家科技支撑计划(2013BAD18B01);国家科技支撑计划(2012BAD12B08); 国家863计划(2011AA100901);

详细信息
    作者简介:

    胡斌 (1984-) , 男, 博士, 研究方向:食品生物技术。;

  • 中图分类号: TS201.3

Extraction of Surface Layer Protein from Lactobacillus acidophilus NCFM and the relationship among SLP and strain acid, bile resistance and cell adhesion

  • 摘要: 对嗜酸乳杆菌NCFM表层蛋白(SLP)的氯化锂(LiCl)提取方法进行了优化,通过SDS-PAGE验证,确定了效率最高的提取条件,即用5mol/L LiCl在35℃处理菌体20min,且提取SLP后,菌体不会失活,8h后重新生成的SLP达到峰值。利用SLP能够再生的性质,分析了SLP与菌体耐酸耐胆盐性能,以及菌体对肠上皮细胞HT-29粘附能力的关系。实验结果显示:随着SLP的再生,去除SLP的菌体酸存活率由0h的52.5%上升到8h后的85.9%,胆盐存活率由0h的43.4%上升到8h后的81.8%,菌体对HT-29细胞的粘附指数从0h的0.33上升到8h后的18.54。SLP再生过程中菌体对酸和胆盐的耐受力和对HT-29细胞的粘附力均有恢复,因此说明SLP的完整对嗜酸乳杆菌NCFM在消化中存活力有重要影响。 
  • [1] 李宗军, 杨秀华, 刘元元, 等.乳杆菌S-层蛋白的多样性及其研究方法[J].食品与生物技术学报, 2009, 28 (6) :721-725.
    [2] Avall-Jaaskelainen S, Palva A.Lactobacillus surface layers and their applications[J].FEMS Microbiology Reviews, 2005, 29 (3) :511-529.
    [3] Altermann, Eric, Russell, et al.Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM[J].Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (11) :3906-3912.
    [4] Goh, Yong Jun, Azcárate-Peril, et al.Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM[J].Applied and Environmental Microbiology, 2009, 75 (10) :3093-3105.
    [5] Konstantinov, Sergey R, Smidt, et al.S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions[J].Proceedings of the National Academy of Sciences, 2008, 105 (49) :19474-19479.
    [6] Buck, B.Logan, Altermann, et al.Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM[J].Applied and Environmental Microbiology, 2005, 71 (12) :8344-8351.
    [7] Sylvie Lortal, et al.S-layer of Lactobacillus helveticus ATCC12046:isolation, chemical characterization and reformation after extraction with lithium chloride[J].Journal of General Microbiology, 1992, 138:611-618.
    [8] B Wang, JS Li, QR Li, et al.Isolation of adhesive strains and evaluation of the colonization and immune response by Lactobacillus plantarum L2 in the rat gastrointestinal tract[J].International Journal of Food Microbiology, 2009, 132:59-66.
    [9] Begley M, Gahan C, G M, et al.The interaction between bacteria and bile[J].Fems Microbiology Reviews, 2005, 29 (4) :625-651.
    [10] 肖荣, 杨秀华, 王远亮, 等.乳酸杆菌的筛选及其S-层蛋白的初步研究[J].农产品加工学刊, 2008, 11:8-10.
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出版历程
  • 收稿日期:  2013-09-04

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