Abstract: The glycoprotein was extracted from defatted cake of Camellia oleifera Abel and purified by column chromatography. Physicochemical property and structural characteristics of the glycoprotein were investigated by color qualitative reaction, isoelectric point (PI) test, SDS-PAGE, β-elimination reaction, fourier transform infrared (FTIR) spectroscopy and automatic amino acid analyzer. The results showed that the glycoprotein was a kind of acidic polysaccharide, its relative molecular weight was 24³6ku and PI value was 3.6. The type of glycopeptide bond oligosaccharide was O-linkage, and glycosidic bond was pyranose α-glycoside linkage type. The glycoprotein by IR analysis showed the typical absorption of polysaccharide and the presence of was indicated. The glycoprotein determined by automatic amino acid analyzer showed the glycoprotein also contained all human essential amino acids and the proportion was appropriate, which was of great value to develop. The physicochemical property and structural characteristics of glycoprotein from Camellia oleifera Abel seed cake was investigated in this paper, which provided theory basis for further research.