Abstract: Response surface methodology (RSM) was used to optimize for ultrasonic-assisted extraction process conditions of essential oil using isopropanol as the solvent from cherry seed. Based on the single factor analysis, the effect of liquid-solid ratio, ultrasonic time, ultrasonic temperature and ultrasonic frequency on enhancing oil yield from cherry seed was evaluated by response surface analysis. Four factors, five levels central composite experimental design was applied and the interactions of each factor on the oil yield were also investigated. The quadratic polynomial regression equation of prediction model was established. Results showed that the liquid-solid ratio, ultrasonic frequency and ultrasonic time had significant effects on extraction yield. Optimal conditions of oil in cherry seed by the ultrasonic-assisted were as follows 1∶8g/mL liquid-solid ratio, 56min extraction time, 40℃ extraction temperature, 110kHz ultrasonic frequency, 300W ultrasonic power. Under such conditions, the oil yield was 12.40%.