Abstract: The microwave-assisted extraction technology of essential oil from citrus flower were optimized using response surface method. The effects of the factors that included extraction agent, the microwave power, the ratio of liquid to solid and the microwave treating time on the yield rate were investigated. The results showed that the optimum microwave-assisted extraction condition was obtained as follows:the extraction agent was n-hexane, microwave power 420W, liquid to solid ratio 6.4∶1 (mL/g) , microwave treating time 185s. Under this optimum condition, the extraction yield of essential oil was 1.04%.