没食子酸清除DPPH自由基的紫外-可见吸收光谱研究

Study on UV-Vis absorption spectrometric investigation of the gallic acid against DPPH free radicals

摘要: 对天然抗氧化剂没食子酸和DPPH自由基的紫外-可见吸收光谱特征进行了研究,确定了没食子酸清除DPPH自由基反应的测定波长（517nm）和反应时间（30min）。进一步研究了没食子酸清除DPPH自由基的活性,获得了没食子酸清除DPPH自由基的半数抑制浓度IC50值。结果表明,没食子酸清除DPPH自由基的IC50值为0.92μg/mL,明显低于芦丁的3.99μg/mL,说明没食子酸具有较高的清除DPPH自由基的活性。

Abstract: The UV-Vis absorption spectrum of gallic acid which is natural antioxidant and DPPH free radicals was investigated, and the determination wavelength (517nm) and the time (30min) for the reaction of gallic acid with DPPH free radicals were optimized. Furthermore, the DPPH free radical scavenging activity of gallic acid was evaluated. And the half inhibitory concentration (IC50) of gallic acid against DPPH was 0.92μg/mL, which was significantly lower than that of standard antioxidant rutin (3.99μg/mL) . The result suggests that gallic acid had high DPPH free radical scavenging activity.