响应面优化酶法辅助提取金针菇根部多糖的工艺研究
Study on optimization of enzymatic extraction of polysaccharide from base of Flammulina velutipes stipe using response surface methodology
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摘要: 目的:对木瓜蛋白酶法辅助提取金针菇根部多糖的工艺进行研究。方法:考察不同酶种类对金针菇根部多糖提取率的影响,选择木瓜蛋白酶用于酶法提取实验研究。在单因素实验的基础上,通过响应面法对影响金针菇根部多糖提取率的三个主要影响因素即酶解pH、酶解温度、酶解时间进行分析优化。结果:影响金针菇根部多糖提取率的工艺因素按主次顺序排列为:酶解温度>酶解时间>酶解pH;确定木瓜蛋白酶酶解金针菇根部多糖最佳工艺条件为木瓜蛋白酶添加量1.5%、料液比1∶20、酶解pH5.5、酶解温度52℃、酶解时间82min。在此最佳条件下,多糖的提取率为5.90%,得率为9.34%,多糖纯度为67.3%,蛋白质含量为5.3%,均极显著优于传统的热水浸提法。结论:采用木瓜蛋白酶辅助提取工艺,相对于传统热水浸提法可显著提高金针菇根部多糖得率和纯度。Abstract: Objective:Polysaccharide in base of Flammulina velutipes stipe was extracted with the assistance of enzymatic hydrolysis.Methods:Papain gave relatively higher polysaccharide yield than cellulase and pectinase, and was chosen for further study.Based on single experiment, a quadratic regression rotational combinational design involving the main three factors at three levels was employed, and data obtained were analyzed with response surface methodology.Results:Polysaccharide yield was most significantly affected by extraction temperature, followed by extraction time and pH.An optimum polysaccharide yield of 5.90%, polysaccharide obtain rate of 9.34% (on the basis of base of F.velutipes stipe weight) , polysaccharide purity of 67.3% and protein content of 5.3% (on the basis of crudue polysaccharide) were obtained when F.velutipes stipe was treated with 1.5% papain at pH5.5, 52℃ for 82min in a material-to-water ratio of 1∶20.Conclusion:The obtain rate and purity of polysaccharide of enzymatic extraction were significantly higher than of conventional hot water extraction.
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