咸鱼中戊糖片球菌产亚硝酸盐还原酶的条件优化
Optimization of conditions of nitrite reductase production in Pediococcus pentosaceus from salted fish
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摘要: 利用从咸鱼中分离出的戊糖片球菌(Pediococcus pentosaceus,P.pentosaceus),对其产亚硝酸盐还原酶(Nitrite Reductase,NiRs)的产酶条件进行优化。单因素实验设计考察接种量、pH、培养温度和培养时间等因素对产酶量的影响,再通过正交实验L9(34)确定了最佳的产酶条件。结果表明,pH对产酶的酶活性影响最大,戊糖片球菌产亚硝酸盐还原酶的最佳产酶条件为接种量4%,pH为6,培养温度为35℃,培养时间为48h。本文为进一步研究亚硝酸盐还原酶性质及在咸鱼加工中应用,以降低咸鱼等腌干鱼类产品中亚硝酸盐及亚硝基化合物提供技术支撑。Abstract: Conditions for nitrite reductase produced in Pediococcus pentosaceus from salted fish were optimized.Based on single factor tests, four factors including inoculate quantity, pH, culture temperature and incubation time were selected as influencing factors by the use of L9 (34) orthogonal experiment design, enzyme activity of nitrite reductase was used as evaluation index.Results showed that the inoculate quantity had greatest influence on enzyme activity.The optimum condition of the Pediococcus pentosaceus nitrite reductase produced were in inoculate quantity of 4%, pH value of 6, culture temperature of 35℃, the incubation time for 48h.It was available for further study of nitrite reductase properties and application in the salted fish processing, in order to reduce the dry salted fish and pickled fish products by nitrite and nitroso compounds to provide technical support.
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